FigureGuild

Multi-Panel Figure Creator

Multi-Panel Figure Creator for Scientific Publications

Combine multiple charts, microscopy images, gels, and diagrams into a single, correctly formatted multi-panel figure. FigureGuild sets journal dimensions, auto-labels panels A–Z, enforces consistent spacing, and exports at the DPI your journal requires.

Create your multi-panel figure →

What is a multi-panel figure?

A multi-panel figure combines two or more data panels (charts, images, diagrams) labeled A, B, C, etc. into a single submission-ready figure file. In most journals, a paper's main figures contain 4–8 panels each, assembled within the journal's column width.

The challenge is consistency: each panel may come from a different source (an R script, a microscope, an imaging software) at a different size and resolution. The Figure Assembler in FigureGuild normalizes all panels to the same physical dimensions, applies uniform spacing and labeling, and produces a single file at the journal's required DPI.

See our complete guide on how to make a multi-panel figure and figure assembly best practices.

Common multi-panel figure layouts

1 × 2 (side-by-side)

Two equal-width panels. Most common for comparing a representative image with a quantification chart.

1 × 3 (three across)

Three equal panels in a row. Common for comparing three conditions or three experimental outcomes.

2 × 2 grid

Four equal panels. Efficient use of full-width space for four related experiments.

Wide + 2 stacked

A large panel (A) with two stacked smaller panels (B, C) to its right. Shows a primary result with two supporting panels.

3-row layout

Six panels in two columns. Common for full-width figures with extensive data.

Custom

Any combination: mix different panel sizes and orientations within the journal column width.

Multi-panel figure rules for publication

  • Panel labels: Uppercase bold A, B, C inside the figure boundary at the top-left of each panel. Minimum 8–10 pt at print size.
  • Spacing: 2–4 mm between all panels, consistent throughout the figure.
  • Color consistency: The same color represents the same experimental group in every panel and every figure in the paper.
  • Font consistency: The same font (Arial/Helvetica) and size for axis labels across all panels.
  • Axis alignment: If panels share an x- or y-axis variable, align the axes to the same range where scientifically appropriate.
  • Scale bars: Required in every microscopy or image panel, labeled with value and unit in the figure legend.

Assemble your multi-panel figure now

Free to start. Journal presets for Nature, Cell, Science, PNAS, eLife, and more.

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